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SEMINARIOS DEL IFLP y DEL DEPARTAMENTO

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Cuándo 15/03/2018
de 11:00 a 12:30
Dónde Aula Chica
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JUEVES 15 de MARZO – 11hs

 

 

TÍTULO: "Magnetic nanoparticles: Current research"

 

Resumen: In this presentation, current results of two research projects of Prof. Dutz (TU Ilmenau/Germany) on the development of magnetic nanoparticles for medical application will be presented.

The first part is on the development of cobalt doped iron oxides. For tuning of the magnetic properties, the Fe2+ ions of magnetite were substituted by Co2+ step by step, which results in a Co doped inverse spinel with adjustable Fe2+ substitution degree. For dry particles, a strong correlation between Co content and resulting Hc and Mr/Ms was found. For increasing Co concentrations from 0 to 8.6% only a slight increase of Hc was found, but from 12 to 25% a strong linear increase results. Within this linear range of dependency, the magnetic properties of the particles, especially Hc, can be tuned easily by changing Co content of the particles. A specific correlation of heating power of gel-immobilized particles with the Co content will be presented and best samples show very good performance for application in hyperthermia.

The second part is on the development of highly biocompatible protein coatings for MNP. For this, cytotoxic polyethylenimine (PEI)-coated nanoparticles were incubated in fetal calf serum (FCS) to form a so-called protein corona on the surface of the particles. The acute cytotoxic effect of incubated particles was tested for human brain microvascular endothelial cells (HBMEC) by the CellTiter Glo™ Cell Viability Assay and for long-term viability up to 96 h by real time cell analysis (RTCA). Interactions of coated MNP and serum incubated MNP with HBMEC were investigated by means of flow cytometry and laser scanning microscopy of fluorochrome-labelled particles. Cell toxicity assays revealed no cytotoxic effect of PEI coated MNP, which were covered by a protein corona. Long-term RTCA showed that the protein corona might mask the cytotoxic effect of PEI. Flow cytometry indicate that FCS coating reduces the particle-cell interaction of cytotoxic PEI coated MNP

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